The culture mainly contains a symbiosis of acetic acid bacteria and one or more yeasts. The culture itself looks somewhat like a large pancake, and though often called a mushroom, a mother of vinegar or by the acronym SCOBY (for "Symbiotic Colony of Bacteria and Yeast"), it is scientifically classified as a zoogleal ma. It takes on the shape of its container, but varies in thickness depending on how long it has been allowed to develop and the acidity of the tea medium during the development period. The culture is leathery and non-elastic, similar to a thick calamari.

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